Miniaturisation pour la Synthèse, l’Analyse et la Protéomique (MSAP) USR 3290


Analysis of yeast and hydrolysats, peptides and proteins

  • Director: Christian ROLANDO (MSAP, Lille)
  • Thesard: Marie YAMMINE

This project aims to the characterization of multiple strains of the yeast Saccharomyces cerevisiae, for the identification and the quantification of proteins and peptides generated by enzymatic and autolytic hydrolysis, and also their post-translational modifications, based on “bottom-up” and “top-down” proteomics approaches and using mass spectrometry. The characterization of the lipidome and the mannoproteins of the yeast cell wall will be included in this project, with a particular interest for the development of methodologies, from sample preparations to bioinformatic analysis

Depolymerization of polymers of biological or synthetic origin by FT-ICR 1D and 2D

  • Director: Christian ROLANDO (MSAP Lille)
  • PhD student: Ziad MAHMOUD

The study of the depolymerization of polymers of biological or synthetic origin is still today a major analytical challenge, particularly with regard to non-soluble polymers. The characterization of polymers and the study of their degradation pathways have an environmental, economic and scientific importance. The objectives of this thesis are:

  • The development of new chemical depolymerization methods specific to each class of polymer using new generations organometallic catalysts,
  • The development of analytical methodologies based on high resolution mass spectrometry for the study of complex mixtures resulting from previous depolymerizations.

These new catalysts make it possible to carry out the degradation reactions of natural or synthetic polymers under milder conditions, further preserving the original structure of the monomers. The new methods of analysis, mainly based on the use of high resolution mass spectrometry including FT-ICR MS on one or two dimensions associated with coupling methods of capillary electrophoresis and ion mobility, will allow to obtain more relevant, rich and detailed structural information.

Generation and trapping of transient species via photochemical and/or electrochemical activation under continuous flow

  • Director: Mael PENHOAT (MSAP Lille)
  • Co-supervisor: Laetitia CHAUSSET-BOISSARIE (MSAP Lille)
  • PhD student: Mélanie ROSEAU

Many of organic processes involve complex reaction pathways and proceed via transient reactive intermediates. Among them, arynes and radicals are classes of reactive intermediates which can provide a prodigious starting point to discover new reactions. Photochemical or electrochemical generation of these transient species can be considered and the use of continuous flow chemistry can circumvent their instability and promote the exploitation of their chemical potential. Moreover, the use of continuous flow chemistry allows the study of in-situ or ex-situ trapping and lifetime species study.